Research on the Life Cycle of Pyrodinium bahamense

Pyrodinium bahamense can threaten public health by contaminating fish and shellfish. Ongoing experiments focus on the organism’s life cycle and will help scientists develop models to predict blooms.

Pyrodinium bahamense in Florida

  Pyrodinium bahamense using electron microscopy
  Pyrodinium bahamense from the Indian River Lagoon viewed using electron microscopy.

Pyrodinium bahamense is a dinoflagellate that blooms regularly in Florida coastal waters, including Tampa Bay, Florida Bay and the Indian River Lagoon.

There are two documented varieties of P. bahamense - one that is generally found in the Pacific Ocean and another in the Atlantic Ocean and Gulf of Mexico. The latter, P. bahamense var. bahamense, is the variety we see in Florida. It has been studied less than the Pacific variety, in part because it was previously not known to be toxic. However, in 2002 scientists confirmed it can produce saxitoxins much like its Pacific counterpart.

Because toxic Pyrodinium blooms can contaminate fish and shellfish and threaten public health, the FWC leads routine monitoring programs for P. bahamense in Tampa Bay and the Indian River Lagoon, the systems in which annual blooms occur. Scientists at the FWC are also working to identify the environmental factors that trigger blooms in Florida and influence their duration, intensity and toxicity. A better understanding of blooms will help scientists develop models to predict future blooms.

Fun Fact

Why Life Cycle Research?

As part of its life cycle, Pyrodinum bahamense forms seed-like cysts, which drop to the seafloor where they overwinter until they are ready to germinate - when optimal conditions arise. Much like with terrestrial plants, successful germination of P. bahamense cysts may determine the timing and intensity of blooms each year. We are currently conducting research to identify the environmental conditions that trigger germination of P. bahamense resting cysts.

 

 

FWC researcher Cary Lopez is currently leading research to address these questions:

  • Do resting cysts require light to germinate?
  • At what temperatures do resting cysts germinate?
  • What environmental conditions favor survival of P. bahamense after germination?
  • What environmental conditions induce Pyrodinium to shift from active growth to cyst formation?
sediment core  Pyrodinium bahamense

Experiments require field collections and laboratory observations. A sediment core taken from Old Tampa Bay (left) is processed for analysis of cyst abundance. Scientists process in subdued green light to minimize light exposure prior to experiments. The emission of red light by Pyrodinium cysts (right) when viewed under blue light indicates that a cyst has either been recently formed or has completed its dormant period and is ready to germinate.


To address these research questions, researchers use a combination of field monitoring and laboratory experiments. Sediment cores have shown abundant living cyst populations in surface sediments of Old Tampa Bay and preliminary laboratory experiments using these cysts indicate that they can germinate and survive for several days in the dark. The ability to germinate in the dark suggests temperature and exposure to oxygen could be more important than light levels for germination. Ongoing laboratory experiments will assess optimal temperature windows for germination and survival after germination.

Pyrodinium bahamense photo series
This photo series captures the Pyrodinium cell as it pops out of its seed-like cyst during germination.

 

 



FWC Facts:
Seagrasses are different from seaweeds (macroalgae) because they have true roots, leaves, internal veins and produce flowers.

Learn More at AskFWC