HAB scientists use two methods of DNA analysis to study HAB and
non-HAB species. The two methods, genetic analysis and molecular
probe analysis, help distinguish similar species.
The HAB group at FWRI studies the DNA
of HAB species using two different research methods, genetic
analysis and molecular probe analysis. Genetic analysis uses
polymerase chain reaction and DNA sequencing to study the
evolutionary relationships between HAB and non-HAB species and to
help identify similar species. Molecular probe analysis uses
fluorescent probes to target the DNA sequence specific to a single
algal species, such as Karenia brevis, the Florida red
tide organism. A species-specific probe decreases the potential for
misidentification in similar species. The HAB group is currently
adapting genetic probes for incorporation into an automated,
near-real-time monitoring system to detect low levels of toxic
algal species in offshore areas. Automatic detection of low levels
of HAB species such as K. brevis will provide early
warning of a potential offshore bloom and, thus, help monitor the
development and movement of blooms.
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